N-acetyl-l-methionine 99%

Identification Test

Take a sample of 250mg, get it dissolved it in 2.5ml isopropyl alcohol and get it diluted with water to 25ml. Take this liquid of 10ml and get it diluted with water to 100m1. Then take the liquid of 0.5,30 and 50μ1 after two dilutions. Drop it at the place with a distance of 2cm from the bottom edge of the thin-layer chromatographic plate. The plate is coated with silica gel (Namely, 20cm * 20cm Brinkman silica gel of 60.250nm, or the equivalent). In a sealed and balanced thin-layered chromatography expansion tank, The chromatography was splayed out by 10cm, and the developer was composed of n-butanol and the mixture of acetic acid and water in the proportion 75：20=20. Dry the plate overnight. Then spray the chromatography plate with a new iodoplatinate solution mixed with 10 percent chloroplatinic acid of 3ml, water of 97ml and 6 percent potassium iodide solution of 100ml. The sample shall be formed - a separate color spot with a Rf value of 0.67~0.1

Toxicity Grade

Safe for food(FDA，§172．372，2000).

Acute Toxicity

Vein - mouse LD50: 435 mg / kg

Use Quantity

Account for 3.1% of the total protein content in food (Calculated by free L- methionine). It shall not be used for infant food and food containing adding nitrite / nitrate (FDA, 172.3722000).

Definition

ChEBI: An L-methionine derivative that is L-methionine in which one of the amine hydrogens is substituted by an acetyl group.

Safety Profile

Poison by intravenous route. When heated to decomposition emits toxic fumes of NOX,.

Purification Methods

Crystallise N-acetyl-L-methionine from Me2CO, H2O or EtOAc. Dry it in a vacuum over P2O5. Its solubility at 25o in H2O is 30.7%, and in Me2CO it is 29.5%. [Mitzi & Schueter Biochim Biophys Acta 27 168 1958, Birnbaum et al. J Biol Chem 194 455 1952, Beilstein 4 IV 3206.]